Search results for "protéines membranaires"

showing 3 items of 3 documents

Gènes fongiques liés au calcium impliqués dans la mycorhize à arbuscules

2012

Fluctuations in intracellular (Ca2+) calcium levels generate signaling events and regulate different cellular processes. Whilst the implication of Ca2+ in plant cell responses during arbuscular mycorrhiza (AM) interactions is well documented, nothing is known about the regulation or role of this secondary meesenger in the fungal symbiont. The molecular basis of fungal calcium homeostasis in the AM symbiosis was analyzed by investigating the expression of Ca2+-related fungal genes. In a first study, G. mosseae genes putatively encoding a MAP3k-like protein kinase (Gm2) and a P-type ATPase (Gm152) were investigated. Both Ca2+-related genes were up-regulated by A. sinicum root exudates, sugges…

Cell signalingGlomus mosseaeHoméostase calcique[SDV]Life Sciences [q-bio]Protéines membranaires/nucléairesCa2+ homeostasiscalcium;gene;fungal;arbuscular mycorrhiza[SDV.BV]Life Sciences [q-bio]/Vegetal BiologyChampignons mycorhizogènesGènes liés au Ca2+thesegeneMembrane/nuclear proteinsMycorrhizal fungi[SDV.SA] Life Sciences [q-bio]/Agricultural sciencescalciumarbuscular mycorrhizaCa2+-related genesTempo-spatial expressionInteractions symbiotiquesSignalisation cellulairefungal[SDE]Environmental SciencesGlomus intraradicesSymbiotic interactionsExpression tempo-spatiale
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Technical improvements for analysis of recalcitrant proteins by LC-MS : the myccorhiza responsive membrane proteome as a case study

2012

Arbuscular mycorrhizas (AM) are widespread symbiotic associations between plant roots and AM fungi. Deep membrane alterations are the foremost morphological changes occurring in the host plant in response to AM symbiosis. Two-dimensional gel electrophoresis (2-DE) is the workhorse method in AM proteomics. Membrane proteins are under-represented in 2-DE because of their hydrophobicity, low abundance, and precipitation at their isoelectric point, thereby few are the identified membrane proteins involved in sustaining the AM symbiosis. Membrane proteomics is still challenging due to 2-DE related shortcomings, however latest trends and advancements in mass spectrometry (MS)-based quantitative p…

Protéomique sans marquageSymbiose mycorhizienne à arbuscules[ SDV.BC ] Life Sciences [q-bio]/Cellular BiologyMedicago truncatula[SDV.BBM] Life Sciences [q-bio]/Biochemistry Molecular BiologyArbuscular mycorrhizas[SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology[SDV.BC]Life Sciences [q-bio]/Cellular BiologyProtéomique hors gelProtéines membranaires[SDV.BC] Life Sciences [q-bio]/Cellular Biology[ SDV.BBM ] Life Sciences [q-bio]/Biochemistry Molecular Biology
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Technical improvements for analysis of recalcitrant proteins by LC-MS : the mycorrhiza responsive membrane proteome as a case study

2012

Arbuscular mycorrhizas (AM) are widespread symbiotic associations between plant roots and AM fungi. Deep membrane alterations are the foremost morphological changes occurring in the host plant in response to AM symbiosis. Two-dimensional gel electrophoresis (2-DE) is the workhorse method in AM proteomics. Membrane proteins are under-represented in 2-DE because of their hydrophobicity, low abundance, and precipitation at their isoelectric point, thereby few are the identified membrane proteins involved in sustaining the AM symbiosis. Membrane proteomics is still challenging due to 2-DE related shortcomings, however latest trends and advancements in mass spectrometry (MS)-based quantitative p…

protéomique hors gel[SDV] Life Sciences [q-bio][SDE] Environmental Sciencesprotéines membranaires[SDV.BV] Life Sciences [q-bio]/Vegetal Biologymedicago truncatulasymbiose mycorhizienne à arbusculesprotéomique sans marquage.
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